王丹
职称:副教授/硕士生导师(无机化学、分析化学)
专业方向:
1) 生物传感器构筑及应用研究
2) 功能蛋白结构及生物效应研究
3) 蛋白纳米材料的构筑及应用研究
联系方式:
E-mail: wangdan1989323@126.com
教育与工作经历:
2007.09---2011.07 广西大学,化学专业,获理学学士学位;
2011.09---2016.12 南京大学,化学专业,获理学博士学位(硕博连读);
2017.02---2021.12 南宁师范大学化学与材料学院,助理研究员;
2021.12---至今 南宁师范大学化学与材料学院,副教授。
教学及指导学生获奖情况:
主讲本科生基础课《无机化学》、《分析化学》和《仪器分析》,其中《分析化学》获批省级一流课程(排名第二)。指导本科生参加广西高校化学化工类论文及设计竞赛并获得一等奖1项,二等奖2项、三等奖3项;指导本科生参加全国大学生生命科学竞赛(2023)获广西赛区三等奖1项,参加广西本科高校第四届生物科学与生物工程类大学生创意、创新和创业竞赛获二等奖3项、三等奖3项;指导本科生参加中国“互联网+”大学生创新创业大赛获得省级铜奖2项,校级一等奖2项、二等奖1项,并获得校级“优秀指导老师”的荣誉称号;指导本科生参加第九届“挑战杯”广西大学生创新创业计划竞赛获得省级铜奖1项,校级三等奖2项;指导本科生参加第三届广西中华职业创新创业大赛暨第四届中华职业教育创新创业大赛获得校级三等奖1项;先后指导14级-19级多名本科生参与“大学生创新创业计划项目”国家级2项,自治区级4项,校级8项。
主持的科研与教改项目:
1. 国家自然科学基金地区科学基金项目,基因编码荧光探针构筑新策略及在重金属胁迫的细胞中毒及解毒机制探究中的应用,项目号:22267015,33万,在研,主持;
2. 国家自然科学基金青年基金项目,蛋白纳米疫苗 VP6-Ferritin 高效免疫的结构基础研究,项目号:31800631,25万,结题,主持;
3. 广西自然科学基面上项目,重金属胁迫的细胞毒理机制研究工具开发及应用的基础研究,项目号:2022JJA120115,10万,在研,主持;
4. 广西自然科学基金青年基金项目,线粒体靶向的汞离子基因编码类荧光探针的设计、构筑及生物成像研究,项目号:2018JJB120049,10万,结题,主持;
5. 2018年度广西高校中青年教师基础能力提升项目,基于蛋白结构的金属结合肽的设计、筛选及应用,项目号:2018KY0361,3万,结题,主持。
6. 2023年自治区级新工科、新医科、新农科、新文科研究与实践项目,基于多学科交叉“政产学研用”赋能的材料化学类专业应用型人才培养体系的构建和实践,项目号:XGK202316,在研,主持.
参与的科研项目:
1. 国家自然科学基金地区科学基金项目,基于靶向近红外碳量子点纳米生物传感器的构筑及其超高灵敏识别病毒核酸的应用基础研究,项目号:21864006,40万,在研,参与;
2. 国家自然科学基金地区科学基金项目,基于碳量子点荧光/磁共振双模态探针的高效构筑及其肿瘤靶向诊疗一体化应用研究,项目号:22164014, 35万元,在研,参与;
3. 国家自然科学基金地区科学基金项目,线粒体作为氧化石墨烯生物效应作用靶点的热力学诠释及其分子机制研究,项目号:21763005,38万元,结题,参与;
4. 国家自然科学基金地区科学基金项目,岩溶环境下地质聚合物材料的溶蚀行为与微结构演变规律, 项目号:51768010,39万元,结题,参与;
5. 广西自然科学基金杰出青年科学基金项目,生物热化学,项目号:2017GXNSFFA198005,60万,结题,参与;
6. 广西自然科学基金重点项目,近红外碳量子点纳米生物传感器的构建及其对生物标志物的超高灵敏检测新方法研究,项目号:2017GXNSFDA198034, 40万,结题,参与;
7. 科技厅科技开发项目广西科技基地和人才专项,基于金纳米簇的自噬诱导剂的制备及其对细胞自噬的调控研究,项目号:桂科AD20297042,在研,参与.
主要学术论文:
2023年
Dan Wang*, Liudan Wei, Jiaxin Tan, Yiyu Yan, Min Wei, Tianyu Song, Sihang Li, Liu Zhao, Weibo Wu, Zhipeng Li*, Qingyou Liu*, A novel strategy of engineering genetically encoded probe for ultrasensitive sensing Hg2+ with unusual planar trigonometric coordination configuration,Analytica Chimica Acta,2023,1252:341049
2022年
Dan Wang, Linwei Duan, Min Wei, Baizhu Chen, Zhipeng Li*, Qingyou Liu*, Establishment of a soluble expression and rapid purification system for self-assembling protein nanoparticle and characterization of its physiochemical properties, Biochemical Engineering Journal, 2022, 186: 108580.
2021年
Yanan Zheng, Liudan Wei1, Linwei Duan, Fangfang Yang, Guixiang Huang, Tianyi Xiao, Min Wei, Yanling Liang, Huiting Yang, Zhipeng Li*, Dan Wang*, Rapid field testing of mercury pollution by designed fluorescent biosensor and its cells-alginate hydrogel-based paper assay, Journal of Environmental Sciences, 2021, 106: 161-170.
2020年
1. Dan Wang*, Yanan Zheng, Xiaosu Fan, Lina Xu, Ting Pang, Ting Liu, Legui Liang, Shan Huang, Qi Xiao,Visual detection of Hg2+ by manipulation of pyocyanin biosynthesis through the Hg2+-dependent transcriptional activator in microbial cells,Journal of Bioscience and Bioengineering, 2020, 129 (2): 223-228
2. Dan Wang*, Yanan Zheng, Liudan Wei, Na Wei, Xiaosu Fan, Shan Huang, Qi Xiao, A signal-amplified whole-cell biosensor for sensitive detection of Hg2+ based on Hg2+-enhanced reporter module,Journal of Environmental Sciences, 2020, 96: 93-98.
3. Shan Huang, Haimei Li, Yi Liu, Liyun Yang, Dan Wang, Qi Xiao*, Investigations of conformational structure and enzymatic activity of trypsin after its binding interaction with graphene oxide, Journal of Hazardous Materials, 2020, 392:122285
2019年
1. Dan Wang*, Yanan Zheng, Lina Xu, Xiaosu Fan, Na Wei, Nanhao Jin, Shan Huang, Qi Xiao, Zihua Wu*,Engineered cells for selective detection and remediation of Hg2+ based on transcription factor MerR regulated cell surface displayed systems,Biochemical Engineering Journal,2019, 150: 107289
2. Zihua Wu*, Yuling Liang, Qing Guo, Keqiu Zhang, Shifang Liang, Liyun Yang, Qi Xiao, Dan Wang*, Study on selective oxidations of gold nanorod and mesoporous silica-coated gold nanorod, Journal of Materials Science, 2019, 54(11) :8133–8147
3. Qi Xiao, Jinrong Feng, Jiawen Li, Yi Liu, Dan Wang, Shan Huang*, Ratiometric electrochemical biosensor for ultrasensitive and highly selective detection of K-ras gene via exonuclease III-assisted target recycling and rolling circle amplification strategies, Analytical Methods, 2019,11, 4146-4156
4. Qi Xiao, Jinrong Feng, Mengmeng Feng, Jiawen Li, Yi Liu, Dan Wang, Shan Huang*, A ratiometric electrochemical aptasensor for ultrasensitive determination of adenosine triphosphate via a triple-helix molecular switch. Microchim Acta, 2019, 186: 478
5. Lina Xu*, Xiaohua Yang, Haiyang Ding, Shouhai Li, Mei Li, Dan Wang, Jianling Xia*, Synthesis of green fluorescent carbon materials using byproducts of the sulfite-pulping procedure residue for live cell imaging and Ag+ ion determination, Materials Science and Engineering: C, 2019,102:917-922
6. Xichun Liu, Qingyuan Hu, Jinmei Yang, Shanqing Huang, Tianbiao Wei, Weizhong Chen, Yafeng He, Dan Wang, Zhijun Liu, Kang Wang, Jianhua Gan, Hao Chen*, Selective cadmium regulation mediated by a cooperative binding mechanism in CadR, Proceedings of the National Academy of Sciences, 2019, 116 (41): 20398-20403
2017年以前
1. Dan Wang, Weizhong Chen, Shanqing Huang, Yafeng He, Xichun Liu, Qingyuan Hu, Tianbiao Wei, Hong Sang, Jianhua Gan, Hao Chen*,Structural Basis of Zn(II) Induced Metal Detoxification and Antibiotic Resistance by Histidine Kinase CzcS in Pseudomonas aeruginosa,Plos pathogens, 2017,13(7):e1006533
2. Dan Wang, Shanqing Huang, Pingying Liu, Xichun Liu, Yafeng He, Weizhong Chen, Qingyuan Hu, Tianbiao Wei, Jianhua Gan, Jing Ma*, Hao Chen*, Structural analysis of the Hg(II)-regulatory protein Tn501 MerR from Pseudomonas aeruginosa, Scientific Reports, 2016, 6: 33391
3. Shanqing Huang, Xichun Liu, Dan Wang, Weizhong Chen, Qingyuan Hu, Wenquan Zhou, Jianhua Gan, Hao Chen*, Structural basis for the selective Pb(II) recognition of metalloregulatory protein PbrR691, Inorganic Chemistry, 2016, 55(24): 12516-12519
4. Xiang Li, Jie Xu, Dan Wang, Ye Sha, Wei Chen, Dongshan Zhou, Xiaoliang Wang, Qing Sun, Gi Xue*, Linling Li*, Low-temperature processing of polymer nanoparticles for bioactive composites, Journal of Polymer Science Part B: Polymer Physics, 2016, 54(24): 2514-2520
5. Weizhong Chen, Dan Wang, Wenquan Zhou, Hong Sang, Xichun Liu, Zhiyun Ge, Jin Zhang, Lefu Lan, Caiguang Yang, Hao Chen*, Novobiocin binding to NalD induces the expression of the MexAB-OprM pump in Pseudomonas aeruginosa, Molecular Microbiology, 2016, 100(5): 5-16
6. Yafeng He, Jian Yuan, Yuchen Qiao, Dan Wang, Weizhong Chen, Xichun Liu, Hao Chen*, Zijian Guo*, The role of carrier ligands of platinum(II) anticancer complexes in the protein recognition of Pt–DNA adducts, Chemical Communications, 2015, 51(74): 14064-14067
7. Yafeng He, Yin Ding, Dan Wang, Wanjun Zhang, Weizhong Chen, Xichun Liu, Weijie Qin, Xiaohong Qian, Hao Chen*, Zijian Guo*, HMGB1 bound to cisplatin–DNA adducts undergoes extensive acetylation and phosphorylation in vivo, Chemical Science, 2015, 6(3): 2074-2078
8. Xing Liu, Xiaoxu Sun, Youcong Wu, Cen Xie, Wenru Zhang, Dan Wang, Xiaoyan Chen, Di Qu, Jianhua Gan, Hao Chen, Hualiang Jiang, Lefu Lan*, Caiguang Yang*, Oxidation-sensing regulator AbfR regulates oxidative stress responses, bacterial aggregation, and biofilm formation in Staphylococcus epidermidis, Journal of Biological Chemistry, 2013, 288:3739-3752
9. Mi Bao, DanWang, Shengyan Liu, Lili Kuang, Jian Sun, Fan Wang*,Yanxuan Wen, Electrodeposition and electrocatalytic activity of Cu2O film on stainless steel substrate, Applied Surface Science, 2012, 258:8008-8014
10. Fan Wang*, Haiqiu Wu, Ziting Lin, Shuaiyuan Han, Dan Wang, Ying Xue, Yunlong Sun, Jian Sun, Bin Li, Shape evolution of Cu-doped Mn3O4 spinel microcrystals: influence of copper content, Materials Research Bulletin, 2010, 45:1567-1573